CLAMP Patch Clamp Amplifier System
The Intan CLAMP system allows users to perform single amplifier or multi-amplifier patch clamp electrophysiology experiments with small, affordable hardware and free, open-source software. This device connects to a host computer through a USB 2.0 cable. Intan digital CLAMP headstages using the revolutionary CLAMP chips (see below) connect to the CLAMP controller via thin, flexible all-digital interface cables.
The CLAMP controller comes in a version with two headstage ports (Part #H1202) and a version with eight headstage ports (Part #H1208). Each CLAMP headstage (Part #H1250) includes a 6-ft (1.8 m) interface cable. Voltage or current measurements are recorded at 50 kSamples/s, and saved data files may be loaded into MATLAB or Python for analysis.
Note: Intan CLAMP software and data formats are not compatible with Clampex or any other third-party patch clamp software.
- Voltage clamp operation with low current noise floor: < 3 pA rms over 5 kHz bandwidth at highest sensitivity.
- Current clamp operation with low voltage noise floor: < 20 μV rms over 10 kHz bandwidth.
- Fast transient capacitance compensation: 0 – 20 pF range; bridge balance in current clamp mode.
(Note: headstages do not have series resistance compensation in voltage clamp mode.)
- Automated measurement of pipette/seal resistance and cell membrane parameters.
- Buzz and zap capability for cell membrane penetration.
- Clamp operation controlled by software and/or external analog voltage command signals.
- Standard threaded Teflon pipette electrode connector is compatible with the 1-HL-U pipette holder from Molecular Devices or the Q Series pipette holders from Warner Instruments.
- May be mounted in standard 19" rack (1U) or operated from a bench top.
More than just a headstage: The Intan CLAMP chips combine all analog patch clamp circuitry and many digital control blocks on a single chip, permitting the construction of Intan-powered digital headstages. A small circuit board containing an Intan CLAMP chip and support components form a complete patch clamp amplifier with a purely digital interface. The digital "headstage" is actually a complete, miniaturized patch clamp amplifier.
Free open-source software: Voltage clamp controls are shown above. A separate Data Display Window shows clamp waveforms and measured voltages and currents in real time. Data may be saved to disk and loaded into MATLAB or Python.
Current clamp demonstration: The intracellular potential of a neuron is monitored during the injection of constant-current pulses.
Voltage clamp demonstration: A cell membrane test performed by applying a voltage step and measuring the resulting whole-cell current.